Hi
I have been preparing libraries from approx 400ng total RNA, mRNA enrichment, using the ScriptSeqV2 kit.
At the end I amplify with 12 cycles and I get a profile with a 2nd peak around 550bp. I have not been able to get an explanation for this yet.
If somebody has an idea I would be grateful.
Thanks Ferdinand
P.S. Profile attached.
I have been preparing libraries from approx 400ng total RNA, mRNA enrichment, using the ScriptSeqV2 kit.
At the end I amplify with 12 cycles and I get a profile with a 2nd peak around 550bp. I have not been able to get an explanation for this yet.
If somebody has an idea I would be grateful.
Thanks Ferdinand
P.S. Profile attached.
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