Dear all,
I am new to NGS and hence i require some help with the analysis of genome sequencing using Ion PGM chip 318.
I have performed a Whole genome shotgun sequencing using Ion PGM on 318 chip. we have generated 331,012,462 bases, 1,307,232 reads and the mean read length is about 253 bp. the genome size is 4,019,665 bp.
I need help to calculate the genome coverage (how much X?)
thanks a lot in advance....
Dr.Pradeep
I am new to NGS and hence i require some help with the analysis of genome sequencing using Ion PGM chip 318.
I have performed a Whole genome shotgun sequencing using Ion PGM on 318 chip. we have generated 331,012,462 bases, 1,307,232 reads and the mean read length is about 253 bp. the genome size is 4,019,665 bp.
I need help to calculate the genome coverage (how much X?)
thanks a lot in advance....
Dr.Pradeep
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