Hi, I am very novice about statistics and data analysis. Recently we have done some RNA sequencing with RNA-tag sequence where we pool many samples in tube for library preperation. We did Hiseq 2500 but after demultiplixing we see huge difference between biological replicate. One experiment looks like this COntrol: A- 1miilion, B-5million, c 0.8 million Treatment: A-3 million, B- 5 million, C 12 million. In a word there is a huge depth variation among biological replicate, i am working with bacteria. Can I use this data for differential expression or should re do the sample. Please help a poor phd!!!!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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