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samtools mpileup to get vcf files including GL information
hello,I use samtools mpileup to call individual snp and indel,my commond lines is samtools mpileup -ugf /ifshk4/PC_HUMAN_US/PROJECT/SZC10013-01_HUMassX/meijunpu/reference/hg18.fa UC5927.mapped.0.bam |../bcftools view -bvcg - |../bcftools view - |../vcfutils.pl varFilter -D 100 > UC5927.mapped.0.var.flt.vcf ,I got the vcf file,but I hope the vcf contains the GL(Likelihoods for RR,RA,AA genotypes (R=ref,A=alt)) information,such as GT:GL 0/1:-323.03,-99.29,-802.53 ,which option I should to add ??? also,how to get GT(genotype),encoded as alleles values separated by “|”, not "/".anyone knows?please help me .
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM