Hi!
I´m initializing in NGS library construction. I will start with Truseq DNA PCR-free kit and I would like to ask you a couple of details. I know people that, following the old protocol runs a Bionalyzer chip after the DNA fragmentation. But in the new protocol, where you have two options for the fragmentation conditions depending on your sequencing purposes, I read you have a cleaning step to be done immediately after the fragmentation, followed by the end repair and size selection steps. Does anyone run the Bioanalyzer in this case?
Based on your experience which are the steps where one could stop the protocol for a few days?
thanks!
I´m initializing in NGS library construction. I will start with Truseq DNA PCR-free kit and I would like to ask you a couple of details. I know people that, following the old protocol runs a Bionalyzer chip after the DNA fragmentation. But in the new protocol, where you have two options for the fragmentation conditions depending on your sequencing purposes, I read you have a cleaning step to be done immediately after the fragmentation, followed by the end repair and size selection steps. Does anyone run the Bioanalyzer in this case?
Based on your experience which are the steps where one could stop the protocol for a few days?
thanks!
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