Is it possible to customise the MiSeq run to do the index read after read 2 (on the read 2 strand). I've seen the Nextera protocol for dual index reads but that's not quite what I'm looking for. Thanks for any advice.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
-
The primers and barcodes for the index read are designed to read immediately after read 1. The primer cannot bind to the reverse complement strand during read 2. If you used your own primers and barcodes, however, you may be able to get around it, but I'm not sure what the advantage of doing that is. Reading the indices at the very end also puts you at risk of not having usable data should the run encounter a problem and stop before the index read.
-
Thanks. Have had to significantly customise our library due to the nature of our work so am already using custom primers and have had to move barcode from it's usual position. Getting good Read 1 and Read 2 but not index. I think I know the issue and doing the index read on the read 2 strand would likely solve it - if it is possible to programme the run to do this. If anyone has done this would be really helpful to know.
Comment
-
Originally posted by PTW View PostIs it possible to customise the MiSeq run to do the index read after read 2 (on the read 2 strand). I've seen the Nextera protocol for dual index reads but that's not quite what I'm looking for. Thanks for any advice.
Comment
-
Originally posted by PTW View PostThanks. Have had to significantly customise our library due to the nature of our work so am already using custom primers and have had to move barcode from it's usual position. Getting good Read 1 and Read 2 but not index. I think I know the issue and doing the index read on the read 2 strand would likely solve it - if it is possible to programme the run to do this. If anyone has done this would be really helpful to know.
Comment
Latest Articles
Collapse
-
by seqadmin
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
Channel: Articles
Yesterday, 07:48 AM -
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 07:17 AM
|
0 responses
11 views
0 likes
|
Last Post
by seqadmin
Yesterday, 07:17 AM
|
||
Started by seqadmin, 05-02-2024, 08:06 AM
|
0 responses
19 views
0 likes
|
Last Post
by seqadmin
05-02-2024, 08:06 AM
|
||
Started by seqadmin, 04-30-2024, 12:17 PM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
04-30-2024, 12:17 PM
|
||
Started by seqadmin, 04-29-2024, 10:49 AM
|
0 responses
28 views
0 likes
|
Last Post
by seqadmin
04-29-2024, 10:49 AM
|
Comment