Hi all,
I would like to combine three 50 reads cartridges (we have half of freezer of expired ones) to get 2x75bp reads. I know it is possible and that it will work and used to have publication where they were making 1200bp reads on MiSeq. But I cannot find it now, so I would like to ask what wells I have to combine and if I have to disassemble the cartridge (not easy) or if I can just puncture the tinfoil and combine them using pasteur pipette.
I would like to combine three 50 reads cartridges (we have half of freezer of expired ones) to get 2x75bp reads. I know it is possible and that it will work and used to have publication where they were making 1200bp reads on MiSeq. But I cannot find it now, so I would like to ask what wells I have to combine and if I have to disassemble the cartridge (not easy) or if I can just puncture the tinfoil and combine them using pasteur pipette.
Comment