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Old 08-29-2013, 03:30 PM   #1
Genohub
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Default DSN-lite, RNase H and Ribo-Zero cook off

I'm curious, has anyone tried the RNAse H method? An elegant method, but you'd have to construct a lot of oligos.

http://www.nature.com/nmeth/journal/...meth.2483.html

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Old 08-29-2013, 05:45 PM   #2
mcnelson.phd
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I've been playing around with the RNase H method, but it's proven a bit finicky for my samples, at least as far as the Bioanalyzer traces show. When it works, it really seems to work well, but I've also had a few test samples become completely degraded.

The method is patented, so I'm hoping that a commercial kit will be put out that supplies the oligos and full-proofs the method a bit more.

Very promising stuff though.
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Old 09-04-2013, 07:25 PM   #3
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Keep me updated if you ever attempt to sequence your RNase H samples. Did you design all the oligos yourself? I'm interested in designing some for mtRNA removal. Yes, a commercial kit would be nice. Anyone know of one coming?

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