Hey guys
Curious as to which system you think is better? I've been trawling this forum regarding size selection using magnetic beads, for use in RADseq (a modified gDNA illumina prep). I'm just a little confused -
- can you buy SPRI beads separately from the technology/hardware? I can't find anything on the beckman page for just SPRI beads. Or am I being dumb and the beads rely on the hardware to work, so you have to buy the whole system for automated library prep?
- are AMPure XP beads (which I have access to) just as good at size selection as SPRI beads? I'm hearing a lot more about the SPRI system on this forum for size selection, rather than the XP beads.
Ideally I would like to size select for fragments 300-500bp, for which I'm aware I'll probably need to do a double selection (one to eradiate fragments >500bp, the other to eradicate fragments <300bp), which is fine.
Also, if anyone knows a good method to calculate the beadNA ratio other than trial and error, that would be greatly appreciated!
Cheers
Tali
Curious as to which system you think is better? I've been trawling this forum regarding size selection using magnetic beads, for use in RADseq (a modified gDNA illumina prep). I'm just a little confused -
- can you buy SPRI beads separately from the technology/hardware? I can't find anything on the beckman page for just SPRI beads. Or am I being dumb and the beads rely on the hardware to work, so you have to buy the whole system for automated library prep?
- are AMPure XP beads (which I have access to) just as good at size selection as SPRI beads? I'm hearing a lot more about the SPRI system on this forum for size selection, rather than the XP beads.
Ideally I would like to size select for fragments 300-500bp, for which I'm aware I'll probably need to do a double selection (one to eradiate fragments >500bp, the other to eradicate fragments <300bp), which is fine.
Also, if anyone knows a good method to calculate the beadNA ratio other than trial and error, that would be greatly appreciated!
Cheers
Tali
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