Is OT1 compatible with my sequencing kit?

[Lotti]

We would like to sequence with Ion PGM Sequencing 200 Kit v2, but we have only OneTouch1 ( and kit for that: Ion OneTouch 200 Template Kit v2 DL). We have 316 v1 and 316 v2 chips too. Could I use OT1 with Ion PGM™ Sequencing 200 Kit v2? Is it compatible? Which chip should I use? v1 or v2?

Are there any serious changes between OT1 and OT2? (e.g. differences between bead sizes or something like this?)

I heard that the polymerase of the Ion PGM Sequencing 200 Kit v2 is only compatible with v2 chips but I am really worried about OT1. Thanks for all the help!

[Smoki]

Officialy, they are not compatible.

In practice, they should work (I already got good results from such combination).

I think the reason that they are not officially listed as compatible is that Life Tech cannot thoroughly test all different combinations.

Hope this helps.