Hi ,
I am working on the RNA-Seq workflow and i am using illumina reads.
I was wondering if i need to perform any kind of QC or read filtering before processing with Tophat tool in the pipeline.
I would like to know what are the different QC practices in use for reads generated from different platform (Illumina, Roche etc)
What type of QC/filter is required to maintain data quality of sequenced reads?
What type of QC does Bowtie & TopHat performs?
Thanks in advance for your valuable suggestions.
I am working on the RNA-Seq workflow and i am using illumina reads.
I was wondering if i need to perform any kind of QC or read filtering before processing with Tophat tool in the pipeline.
I would like to know what are the different QC practices in use for reads generated from different platform (Illumina, Roche etc)
What type of QC/filter is required to maintain data quality of sequenced reads?
What type of QC does Bowtie & TopHat performs?
Thanks in advance for your valuable suggestions.