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  • Hi from a downstream user of NGS data

    Hello,

    I'm a curator/data wrangler for a large NIH-funded sequencing consortium. Part of my job is making sure that we collect enough information about the experiments so that others can re-use the data as much as possible. Additionally I work with the data itself, doing QC, etc. So, I do not use the data for analysis, per se; rather i help to make the data available to others so they can analyze it! For the most part, I use SAMtools and the various genome browsers that display the data; I have not used the specific software or equipment that produce the reads themselves.

    This forum will help me stay on top of the issues that seem to be facing the experimentalists, and will help me understand any problems associated with this rapidly progressing technology!

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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