Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Run error - no clustering

    Hi,
    I ran a MiSeq run last week and the run was aborted due to clustering failure.
    I am not sure whether it is due to the library preparation, reagents or the machine. My run is a 2x250 cycle run and I loaded 8pm of the library in with 8% Phix spike-in.

    I have worked with the first 2 panels of primers without any problems and added in Panels 3 to 6. Below is the number of primers I have for each panel.

    - Panel 1 – 12 forward, 12 reverse
    - Panel 2 – 14 forward, 12 reverse (same reverse as Panel 1)
    - Panel 3 – 68 forward, 26 reverse
    - Panel 4 – 8 forward, 4 reverse
    - Panel 5 – 14 forward, 6 reverse
    - Panel 6 – 14 forward, 30 reverse

    Would appreciate any comments/help on this! Thank you!

  • #2
    Your best bet is to contact Illumina tech support. They can look at your instrument remotely and diagnose the failure. Illumina will replace the kit if they determine that the failure was due to instrument/reagents (you will need a maintenance contract for this but most people do have one).

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    10 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    51 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X