Hi,
I ran a MiSeq run last week and the run was aborted due to clustering failure.
I am not sure whether it is due to the library preparation, reagents or the machine. My run is a 2x250 cycle run and I loaded 8pm of the library in with 8% Phix spike-in.
I have worked with the first 2 panels of primers without any problems and added in Panels 3 to 6. Below is the number of primers I have for each panel.
- Panel 1 – 12 forward, 12 reverse
- Panel 2 – 14 forward, 12 reverse (same reverse as Panel 1)
- Panel 3 – 68 forward, 26 reverse
- Panel 4 – 8 forward, 4 reverse
- Panel 5 – 14 forward, 6 reverse
- Panel 6 – 14 forward, 30 reverse
Would appreciate any comments/help on this! Thank you!
I ran a MiSeq run last week and the run was aborted due to clustering failure.
I am not sure whether it is due to the library preparation, reagents or the machine. My run is a 2x250 cycle run and I loaded 8pm of the library in with 8% Phix spike-in.
I have worked with the first 2 panels of primers without any problems and added in Panels 3 to 6. Below is the number of primers I have for each panel.
- Panel 1 – 12 forward, 12 reverse
- Panel 2 – 14 forward, 12 reverse (same reverse as Panel 1)
- Panel 3 – 68 forward, 26 reverse
- Panel 4 – 8 forward, 4 reverse
- Panel 5 – 14 forward, 6 reverse
- Panel 6 – 14 forward, 30 reverse
Would appreciate any comments/help on this! Thank you!
Comment