Hello all,
I want to do Illumina miseq on total DNA extracted from feacal microbes to check the relative abundance of different microflora.
Problem is that i am getting very low DNA concentration the samples i am using to extract DNA, I am using Qiagen stool DNA extaction kit and the concentration i am getting on Nanodrop 2000 is very low ( I.e. less than 10 ng/ul in most of the sample).
I have also performed gel electrophresis to check the molecular band of my total DNA, and the band i am getting is very shallow against Hind II Lamda marker.
I want to know if that concentration is enough to do PCR amplification for the Illumina Miseq to get the sequencing library ?
Regards,
Umair
I want to do Illumina miseq on total DNA extracted from feacal microbes to check the relative abundance of different microflora.
Problem is that i am getting very low DNA concentration the samples i am using to extract DNA, I am using Qiagen stool DNA extaction kit and the concentration i am getting on Nanodrop 2000 is very low ( I.e. less than 10 ng/ul in most of the sample).
I have also performed gel electrophresis to check the molecular band of my total DNA, and the band i am getting is very shallow against Hind II Lamda marker.
I want to know if that concentration is enough to do PCR amplification for the Illumina Miseq to get the sequencing library ?
Regards,
Umair
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