Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • what's wrong with heatmap.2, heatmap turns to blue after finishing plotting

    Hi everyone,

    I was plotting a transcription factor ChIP-seq data at TSS with heatmap.2



    the problem is that the plot becomes blue after plotting as the green-red colour setting.
    if you resize the window, you can see the red signal enriched at the TSS, but once finished plotting, the blue spread to the whole figure and I could not see anything...

    See the picture above.

    what's wrong with heatmap.2?

  • #2
    I don't think this is a problem of the heatmap function, but more of your system. It seems that your data matrix is quite huge, so may I ask on what kind of hardware you are creating this heatmap? It may be that your RAM is too small or your graphics card simply cannot handle this much output.
    As a possible solution: have you tried to save the plot to file directly and to open the file afterwards?

    Comment


    • #3
      Originally posted by rboettcher View Post
      I don't think this is a problem of the heatmap function, but more of your system. It seems that your data matrix is quite huge, so may I ask on what kind of hardware you are creating this heatmap? It may be that your RAM is too small or your graphics card simply cannot handle this much output.
      As a possible solution: have you tried to save the plot to file directly and to open the file afterwards?
      yes, I tried to save it first, and after open it, it is still covered by blue.

      The matrix is somewhat large, with 1728 rows and 601 columns, but many of them are 0s.

      computer info:
      Ubuntu 12.04 (precise)
      Intel(R) Core(TM) i5-2520M CPU @ 2.50GHz
      4G memory

      I will try to run it on High performance cluster in our school to see if it has the same problem. Thanks!

      Comment


      • #4
        solved in Biostar.

        I need to put the trace="none" when I plot the heatmap
        Thanks.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM
        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        27 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        31 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        27 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-04-2024, 09:00 AM
        0 responses
        52 views
        0 likes
        Last Post seqadmin  
        Working...
        X