Hello everybody!
I have a question about the calibrated quality string in qseq.txt file.
If I have full called sequence BUT a calibrated quality string full of “B” and the read passes the filtering (1), I should discard this read (although passed the filter)?
And if I have two reads in which the first read didn’t passed the filter while the second read passed BUT the number of “B” within is similar,
which read should I discard? Otherwise I could cut the read after “B” appeared?
In a few words, how I must to consider the “B” score for the next anlysis??
I have a question about the calibrated quality string in qseq.txt file.
If I have full called sequence BUT a calibrated quality string full of “B” and the read passes the filtering (1), I should discard this read (although passed the filter)?
And if I have two reads in which the first read didn’t passed the filter while the second read passed BUT the number of “B” within is similar,
which read should I discard? Otherwise I could cut the read after “B” appeared?
In a few words, how I must to consider the “B” score for the next anlysis??
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