Hello All,
We have prepared illumina Mate pair libraries using the standard 2-5 Kb protocol. But one major limitation for illumina mate pair libraries has been there read length. We only can sequence up to 36 bases of a read and can't go higher due to technical difficulties in the protocol (there is every possibility we may end up sequencing in to the other end of the Biotin which ends up giving lots of chimeras). For our analysis, A 36 base read length is not ideal especially if your assembling Homologous Genomes or regions.
Has someone developed a mate pair library protocol where you can sequence up to 100 bases in length without much trouble. I know BGI has one, developed at there facility. But please let me know.. if any one are aware of other than this.
Best,
Rakesh
We have prepared illumina Mate pair libraries using the standard 2-5 Kb protocol. But one major limitation for illumina mate pair libraries has been there read length. We only can sequence up to 36 bases of a read and can't go higher due to technical difficulties in the protocol (there is every possibility we may end up sequencing in to the other end of the Biotin which ends up giving lots of chimeras). For our analysis, A 36 base read length is not ideal especially if your assembling Homologous Genomes or regions.
Has someone developed a mate pair library protocol where you can sequence up to 100 bases in length without much trouble. I know BGI has one, developed at there facility. But please let me know.. if any one are aware of other than this.
Best,
Rakesh
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