Hi All,
I have alignment files from RNA-seq data in .sam format. I want to calculate read counts and RPKM values using RefSeq reference data (refseq.gft) file for several samples. I tried to use HTSeq, TopHat etc but I have not be able to get RPKM and read counts. Can you please suggest appropriate bioinformatics tools?
Thanks for your help,
Rakesh
I have alignment files from RNA-seq data in .sam format. I want to calculate read counts and RPKM values using RefSeq reference data (refseq.gft) file for several samples. I tried to use HTSeq, TopHat etc but I have not be able to get RPKM and read counts. Can you please suggest appropriate bioinformatics tools?
Thanks for your help,
Rakesh
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