Hi
I have been processing some 150bp paired end Nextera XT reads from viral cDNA. The first 20 or so bases at the 5'end are due to Nextera which I just trim off, but I also have an unusual base content at the 3'end of all sequences, consistently in all samples. I have attached the fastqc images for the forward reads of one sample, which is representative of all my samples. Does anyone know what this might be?
I have been processing some 150bp paired end Nextera XT reads from viral cDNA. The first 20 or so bases at the 5'end are due to Nextera which I just trim off, but I also have an unusual base content at the 3'end of all sequences, consistently in all samples. I have attached the fastqc images for the forward reads of one sample, which is representative of all my samples. Does anyone know what this might be?
Comment