SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
How are you liking your NextSeq 2000? mikerobiology Illumina/Solexa 0 12-01-2020 09:55 AM
Problems sequencing a low diversity library on NextSeq aasako Illumina/Solexa 8 09-17-2020 06:02 AM
NextSeq 1000 and 2000 cluster density and number of copies Dornfield Illumina/Solexa 4 02-28-2020 11:12 AM
Index read Q30 problems on NextSeq Vesperholly2 Illumina/Solexa 5 11-06-2015 04:32 AM
N50 less than 2000 sarbashis Illumina/Solexa 4 09-07-2011 04:06 AM

Reply
 
Thread Tools
Old 05-28-2021, 07:44 AM   #1
seqa_van
Junior Member
 
Location: Boston, MA

Join Date: May 2021
Posts: 1
Default Nextseq 2000 problems?

Hello,

I run a small core facility and we recently traded in our Hiseq 2500 for a Nextseq 2000 and since receiving the instrument ~2 months ago it has been down more than it has been up. Illumina has replaced the compute engine twice, the internal dongle with software authentication keys (which arrived mismatched), and the monitor as well as troubleshooting multiple software and hardware issues that prevented us from starting runs, integrating with basespace for run perfomance monitoring, and performing secondary analysis. We are now experiencing multiple GPU errors leading to the instrument screen going black and non-responsive from an idle state and then hanging on bootup. Illumina seems mystified (but hasn't offered to replace the instrument) so at this point I'm wondering if other people are experiencing these issues? I would appreciate hearing other's experienced with the NExtseq 2000; good or bad! TIA!
seqa_van is offline   Reply With Quote
Old 05-28-2021, 08:00 AM   #2
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,132
Default

Sorry to hear about the problems you are having. This seems unusual. Since it is a brand new machine it will get fixed/replaced but you are losing time.

We just got first NS 2K (we have all other types) installed and hope to start using it next week. No red flags so far during installation/validation.

Last edited by GenoMax; 05-28-2021 at 08:03 AM.
GenoMax is offline   Reply With Quote
Old 06-10-2021, 08:44 AM   #3
misterc
Member
 
Location: Livermore, CA

Join Date: Jan 2016
Posts: 46
Default

Sorry to hear about the multiple repairs on this instrument. If it were my instrument, I'd already be fighting hard to have the entire unit replaced or at the very least have them provide a free loaner instrument for a few months.

We've been using our NextSeq 2k for about 5 months now, and haven't had any hardware or software failures.
misterc is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:55 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO