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Old 09-18-2009, 03:54 PM   #1
greigite
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Location: Cambridge, MA

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Default read length distributions?

Hi,
I'm looking for information on the typical distribution of read lengths and error rates in an Illumina run, assuming a target length of 76 bp. Could anyone point me to some tabular data on this (not just a figure in a paper)? We are trying to create a simple simulation model to test assembly of a mixed community using different read lengths and need to know these parameters.
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Old 12-01-2009, 08:42 AM   #2
PeterGoe
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Default

Quote:
Originally Posted by greigite View Post
Hi,
I'm looking for information on the typical distribution of read lengths and error rates in an Illumina run, assuming a target length of 76 bp. Could anyone point me to some tabular data on this (not just a figure in a paper)? We are trying to create a simple simulation model to test assembly of a mixed community using different read lengths and need to know these parameters.
I would also be interested in any information on average error rates (per cycle) within a 75 bp, paired end PhiX run, as this would be very helpful in building a simple error model for a metagenomic read simulation.

Cheers.
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