SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Why and how are gDNA contaminations in RNAseq library preps bad? lukas1848 Sample Prep / Library Generation 1 11-04-2011 04:51 AM
Library prep can you get both mRNA and small RNAs in the same library? willgordon Sample Prep / Library Generation 4 07-20-2011 12:31 PM
Very Bad Mapping Results with several mapping softwares xquan Bioinformatics 13 05-23-2011 12:31 AM
A really bad library? FrSr RNA Sequencing 2 02-11-2011 02:45 AM
ChIP library prep results (Illumina) theduke Sample Prep / Library Generation 9 12-09-2010 02:16 PM

Reply
 
Thread Tools
Old 07-25-2010, 01:39 AM   #1
dina
Member
 
Location: israel

Join Date: Sep 2009
Posts: 34
Default bad library prep results

Hi,
Has anyone encountered a problem in the rapid protocol of 454? We have a good cDNA, but after the rapid protocol nothig is left,,,,
Thank you!
dina is offline   Reply With Quote
Old 08-04-2010, 06:42 AM   #2
v_kisand
Member
 
Location: Eesti

Join Date: Jan 2009
Posts: 37
Default

meaning no library or later no reads when sequencing?

v.
v_kisand is offline   Reply With Quote
Old 08-18-2010, 04:10 AM   #3
dina
Member
 
Location: israel

Join Date: Sep 2009
Posts: 34
Default library

hi
sorry for the late reply, I was on a vacation. I ment we receive very low concentrations of cDNA immediately after the rapid protocol
dina is offline   Reply With Quote
Old 09-16-2010, 06:00 AM   #4
v_kisand
Member
 
Location: Eesti

Join Date: Jan 2009
Posts: 37
Default

no clue in this case, sry...
v_kisand is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:03 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO