Hi all,
I was trying to pool my ChIP DNAs into one for library construction using the AMPure XP beads (300 ul sample + 1.6x beads).
However, after adding 31 ul of TE for elution, I recovered around 37 ul AND it looks a bit cloudy.
I did try repeating the XP purification once more (37 ul of eluates + 1.6x beads) but got similar results.
I suspect the cloudiness is from the PEG-containing buffer of the beads, which somehow hasn't been efficiently removed.
My main question is whether this "cloudiness" would interfere with later steps in library prep (next step would be Ends Repair) or it'd be better to remove it by MinElute.
Any inputs on how to avoid this are also very welcome.
Thank you!
osl
I was trying to pool my ChIP DNAs into one for library construction using the AMPure XP beads (300 ul sample + 1.6x beads).
However, after adding 31 ul of TE for elution, I recovered around 37 ul AND it looks a bit cloudy.
I did try repeating the XP purification once more (37 ul of eluates + 1.6x beads) but got similar results.
I suspect the cloudiness is from the PEG-containing buffer of the beads, which somehow hasn't been efficiently removed.
My main question is whether this "cloudiness" would interfere with later steps in library prep (next step would be Ends Repair) or it'd be better to remove it by MinElute.
Any inputs on how to avoid this are also very welcome.
Thank you!
osl
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