Dear All,
1) I have been working to code for a tool to get the digital gene expression count for genes across different samples. I wanted to know that can the Digital gene expression level be taken as the number of reads mapping to that gene?
If yes, then is that an accurate way of estimation of getting the expression level without going for a separate experiment to find the levels?
2) Also, I have heard that using cufflinks coverage, we can derive at a count for a gene - is that true? I found it confusing as the coverage values provided by the software, is observed always in decimals as 0.007 or may be 1.05. How can a gene be expressed in such terms? Am i missing some logic here?
Will be glad to receive some answers for the above queries.
Thanks in advance!
1) I have been working to code for a tool to get the digital gene expression count for genes across different samples. I wanted to know that can the Digital gene expression level be taken as the number of reads mapping to that gene?
If yes, then is that an accurate way of estimation of getting the expression level without going for a separate experiment to find the levels?
2) Also, I have heard that using cufflinks coverage, we can derive at a count for a gene - is that true? I found it confusing as the coverage values provided by the software, is observed always in decimals as 0.007 or may be 1.05. How can a gene be expressed in such terms? Am i missing some logic here?
Will be glad to receive some answers for the above queries.
Thanks in advance!
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