Hi,
our lab is going to resequence the whole genome of a Drosophila melanogaster mutant strain using HiSeq. I found that Qiagen's Gentra Puregene and Genomic-tip are both used in literature for the genomic DNA preparation. Dose anyone have the experiences about which one gives better quality of the important starting materials for the downstream library preparation, sequencing... etc? Apart from the two kits, any protocols/kits that give good quality of Drosophila gDNA for next generation sequencing are all very appreciated.
Best regards,
Sue
our lab is going to resequence the whole genome of a Drosophila melanogaster mutant strain using HiSeq. I found that Qiagen's Gentra Puregene and Genomic-tip are both used in literature for the genomic DNA preparation. Dose anyone have the experiences about which one gives better quality of the important starting materials for the downstream library preparation, sequencing... etc? Apart from the two kits, any protocols/kits that give good quality of Drosophila gDNA for next generation sequencing are all very appreciated.
Best regards,
Sue
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