Hi, we have been considering about using HiSeq 2500 to sequence ~100 cancer genomes. We were wondering why the Rapid Run mode saves so much time compared to the High Output mode (500 cycles in < 3 days versus 250 cycles in 6 days, if I understand correctly). Since the chemical procedure for each cycle seems to be quite similar in both mode (nucleotide incorporation -> wash -> imaging -> remove blocking and fluor group -> wash) and all reactions happen in a parallel manner, where comes the substantial difference in run time?
Many thanks in advance!
Many thanks in advance!
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