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Old 08-03-2011, 06:01 AM   #1
chaos81
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Location: miami

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Default covaris shearing range

I was wondering if anyone has used the covaris for a shearing range of 2-5kb for DNA and what were your results. I need this size range to start a protocol and want to know if the machine has the capabilities to perform this.
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Old 08-14-2011, 05:20 PM   #2
ScottC
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Location: Monash University, Melbourne, Australia.

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Default

Yes, it works reasonably well if you have sufficient starting material. The range of fragments is relatively wide at large size targets, so you will lose a reasonable amount of your DNA. Check their webpage for protocols to shear DNA to 5kb. They also produce special tubes for various kb size ranges.

Cheers,

Scott.
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