Hello all,
When you do a solexa run, how many reads can you obtain using for example 1 lane (let's say for reads of 35 bp or 50 bp)? And more important, how many of these reads are useful, meaning that they don't contain any adapter/primer sequence?
Does it also differ depending on the type of analysis you want to perform (RNASeq, WGS, Gene enrichment, ...) or do you always see the same amount of reads containing adapter sequences?
I ask this because in certain datasets, we see that almost 2/3 of the reads contain an adapter sequence, which is huge.
Thanks for the comments,
Steven
When you do a solexa run, how many reads can you obtain using for example 1 lane (let's say for reads of 35 bp or 50 bp)? And more important, how many of these reads are useful, meaning that they don't contain any adapter/primer sequence?
Does it also differ depending on the type of analysis you want to perform (RNASeq, WGS, Gene enrichment, ...) or do you always see the same amount of reads containing adapter sequences?
I ask this because in certain datasets, we see that almost 2/3 of the reads contain an adapter sequence, which is huge.
Thanks for the comments,
Steven
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