SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
looking to outsource DNA shearing delbar Core Facilities 16 10-25-2012 07:48 PM
g-TUBE for DNA Shearing coffee52 Sample Prep / Library Generation 0 02-06-2012 10:46 PM
Shearing DNA for Chip-seq macgabhain Epigenetics 7 09-15-2010 10:59 AM
DNA shearing suludana Illumina/Solexa 18 03-01-2010 06:30 PM
DNA Shearing for 454 alanc 454 Pyrosequencing 3 02-26-2009 02:27 AM

Reply
 
Thread Tools
Old 11-07-2008, 01:12 AM   #1
alanc
Junior Member
 
Location: Netherlands

Join Date: Nov 2008
Posts: 3
Default New DNA shearing technology?

I came across this technology for DNA shearing http://www.kbioscience.co.uk/instrum...-DNA_shear.htm Has anyone got any experience with this technology?
alanc is offline   Reply With Quote
Old 11-07-2008, 05:19 AM   #2
andibody
Member
 
Location: Vienna

Join Date: Mar 2008
Posts: 45
Default

Hi,

We are successfully using a Covaris sonicator to shear DNA for ChIP-Seq on Solexa platform.
Especially the reproducibility is higher than that of other systems we have tested so far.
Only the cost price is a major drawback.

Best
andibody
andibody is offline   Reply With Quote
Old 11-07-2008, 06:40 AM   #3
Chipper
Senior Member
 
Location: Sweden

Join Date: Mar 2008
Posts: 324
Default

Well, buy a SOLiD then, and you will get a Covaris also. The other drawback is throughput I guess, the Bioruptor takes up to six samples at once.
Chipper is offline   Reply With Quote
Old 11-07-2008, 08:15 AM   #4
alanc
Junior Member
 
Location: Netherlands

Join Date: Nov 2008
Posts: 3
Default

I did see they now have a 96well version as well. I'm slightly worried that the biorupters sonication heats the sample and we could get biased shearing. On the Covaris website they state that this method is isothermal. Did anyone check this yet?
alanc is offline   Reply With Quote
Old 07-09-2011, 08:25 AM   #5
elena.or
Junior Member
 
Location: Germany

Join Date: Jul 2011
Posts: 2
Default KASPar Kbioscience

Hallo,
does anybody have experience with the KASPar genotying assay from Kbioscience?
http://www.kbioscience.co.uk/reagents/KASP/KASP.html

I am thinking of using this technology but I first would like to know your experience.
Thanks a lot!
elena.or is offline   Reply With Quote
Old 07-25-2012, 06:53 AM   #6
TPayette
Junior Member
 
Location: Golden, CO

Join Date: May 2012
Posts: 1
Default

I'm looking to create a couple of 8kb paired end rapid libraries for 454 seq and don't have a HydroShear, I realize there are a few places to outsource the shearing step to facilities such as Profile Genomics or Covaris but has anyone tried Covaris's g-TUBE single-use device that shears genomic DNA into selected fragments sizes ranging from 6kb to 20kb. The only equipment needed is a compatible bench-top centrifuge, looks like it would be about $27/sample.
TPayette is offline   Reply With Quote
Reply

Tags
covaris, dna shearing, illumina, solexa

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:51 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO