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Old 05-15-2012, 08:40 AM   #1
durandg
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Location: Lyon, France

Join Date: May 2012
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Default Ideas for shearing and/or size-selected FFPE DNA?

Hello,

I want to perform exome sequencing on a SOLID 5500 with DNA extracted from FFPE.

After a Bioanalyzer run, i've seen -of course- a range of sizes up to Kb.
Even with doing a shearing and a size selection using the standard protocol of Library prep (Life Tech), i select sizes that are bigger that what i usually get, and actually too long to perform a correct run, for sure.

Does anyone have an idea of bead ratios to use or any program for the Covaris S220 to shear efficiently that kind of degraded DNA

All help will be appreciated :-)

Regards,

Durandg.
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