Hello
I have sequenced a BAC clone with PacBio RSII
To make the assembly I used Facon through pbbioconda and for polishing I used quiver
To have an estimation of the consensus quality I re map the original bam reads file against the consensus
How to estimate a mean quality value, in other world a consensus Phred score for the base calls of the consensus ... :-)
Thank you in advance
Philippe
I have sequenced a BAC clone with PacBio RSII
To make the assembly I used Facon through pbbioconda and for polishing I used quiver
To have an estimation of the consensus quality I re map the original bam reads file against the consensus
How to estimate a mean quality value, in other world a consensus Phred score for the base calls of the consensus ... :-)
Thank you in advance
Philippe
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