Hi Friends
If I plan to clone RNA near its 3' end and somehow select just the 3' ends. What do you think about clusters when sequencing the A tail. I am planning to sequence from the back-end of the RNA. Will the clusters be unique or because every spot will glow for A (or T) it might confuse with the clusters?
Does anyone have idea whether we can place a barcode before the insert and read it as a part of insert? Any alternatives?
Please share your experiences and suggest some other ideas?
Biochembug
If I plan to clone RNA near its 3' end and somehow select just the 3' ends. What do you think about clusters when sequencing the A tail. I am planning to sequence from the back-end of the RNA. Will the clusters be unique or because every spot will glow for A (or T) it might confuse with the clusters?
Does anyone have idea whether we can place a barcode before the insert and read it as a part of insert? Any alternatives?
Please share your experiences and suggest some other ideas?
Biochembug
Comment