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  • phylogenetic analysis with stacks using RAD-seq

    Hi all,

    I am trying to do phylogenetic analysis using Stacks --phylip option it says no data to write in phylip

    Generating nucleotide-level summary statistics for population '1'
    Population '1' contained 0 incompatible loci -- more than two alleles present.
    Generating nucleotide-level summary statistics for population '2'
    Population '2' contained 0 incompatible loci -- more than two alleles present.
    Tallying loci across populations...done.
    Pruned 264 variant sites due to filter constraints (too many alleles or too few samples at a site, MAF too low, or heterozygosity too high).
    Removing 253 additional loci for which all variant sites were filtered... retained 2785 loci.
    Regenerating nucleotide-level summary statistics for population '1'
    Population '1' contained 0 incompatible loci -- more than two alleles present.
    Regenerating nucleotide-level summary statistics for population '2'
    Population '2' contained 0 incompatible loci -- more than two alleles present.
    Re-tallying loci across populations...done.
    Generating haplotype-level summary statistics for population '1'
    Generating haplotype-level summary statistics for population '2'
    Writing 2785 loci to summary statistics file, './trial/batch_1.sumstats.tsv'
    Writing 2785 loci to observed haplotype file, './trial/batch_1.haplotypes.tsv'
    Writing population data to Phylip file './trial/batch_1.phylip'; logging nucleotide positions to './trial/batch_1.phylip.log'... No data is available to write to the Phylip file.

    Please help

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