Hi, maybe it is a trivial question but I did not find a satisfactory answer at the forum. How can you decide by score or other evaluation values whether a hit is unique or not?
I have this example (from BLAT output):
Is that a unique hit because the matches of the first hit are much more than the matches of the second hit?
For example, is there a rule that you can say the hit is unique because the score of the second best hit is lower than 90 % of the best hit score or something?
I have this example (from BLAT output):
matches
query size
chromosome
2440
2524
11
31
2524
10
Is that a unique hit because the matches of the first hit are much more than the matches of the second hit?
For example, is there a rule that you can say the hit is unique because the score of the second best hit is lower than 90 % of the best hit score or something?
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