Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • ARDEN gives wrong sequence result

    Hi,

    I am using ARDEN to make artificial sequence from the original one which is 2.5 GB fasta file the result file is only 500 kb.

    This is the command I am using
    Code:
     python arden-create ./genomeTest/ /data/qdata/public/illumina-genomes/Zea_mays/Ensembl/AGPv2/Sequence/Bowtie2Index/genome.fa -d 21 -o 0 -n maize
    and print out in the terminal

    ########Some stats:########
    DNA length: 569630
    max substitutions: 27125
    #Balanced Mutations: 9206
    #hamming distance REF-ART 18412
    avg. distance: 30.9379752335
    ###########################

    any idea


    Edit:
    The answer to my question is ARDEN only works with one chromosome per time so i can not run it on the whole chromosomes "one file contains the whole genome" at once.
    Last edited by Medhat; 11-19-2013, 05:10 AM.

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
22 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
46 views
0 likes
Last Post seqadmin  
Working...
X