Hi,
I am trying to convert MiSeq .bcl files into uSAM with Picard tools 'CheckIlluminaDirectory', 'ExtractIlluminaBarcodes' and 'IlluminaBasecallsToSam'.
These commands require to provide the READ_STRUCTURE option (described here)
Do you have any idea what the READ_STRUCTURE would be, knowing that I made a library with a TruSeq Exome kit (6 plex) and sequenced it on a MiSeq (2 x 75 bp - 76 cycles)?
Can we get this info from some of the sequencer run log files maybe?
I am trying to convert MiSeq .bcl files into uSAM with Picard tools 'CheckIlluminaDirectory', 'ExtractIlluminaBarcodes' and 'IlluminaBasecallsToSam'.
These commands require to provide the READ_STRUCTURE option (described here)
Do you have any idea what the READ_STRUCTURE would be, knowing that I made a library with a TruSeq Exome kit (6 plex) and sequenced it on a MiSeq (2 x 75 bp - 76 cycles)?
Can we get this info from some of the sequencer run log files maybe?
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