Hi Members,
I've lost, yet again with direction, and sequencing. I'm working on 16s data. Paired end, Illumina reads. And since then I am confused over the output in read 2- fastq.gz
5'------>primer SEQUENCE END 3'
3' END SEQUENCE PRIMER<-------5'
Paired end sequencing as per my understanding looks like above. Primer or index, or bar code, adapter, Pardon me for not using appropriate terms.
My doubt:
1) In the fastq of reverse strand, how do I have the output:
END.............SEQUENCE
OR
SEQUENCE......END
I'm confused over the direction in which I have the fastq output.
I've lost, yet again with direction, and sequencing. I'm working on 16s data. Paired end, Illumina reads. And since then I am confused over the output in read 2- fastq.gz
5'------>primer SEQUENCE END 3'
3' END SEQUENCE PRIMER<-------5'
Paired end sequencing as per my understanding looks like above. Primer or index, or bar code, adapter, Pardon me for not using appropriate terms.
My doubt:
1) In the fastq of reverse strand, how do I have the output:
END.............SEQUENCE
OR
SEQUENCE......END
I'm confused over the direction in which I have the fastq output.
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