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Old 03-06-2012, 02:06 AM   #1
andyding
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Question Maximum Insert Size of Illumina Libraries?

Hi, all:
I'm wondering what is the maximum insert size of illumina paired-end or mated-paired libraries? Some source saids it's 20kb or 40kb, I'm wondering if it is possible that I can get a even longer insert size?
Moreover, will library preparing process become more complicated or harder when the insert size increases?
Thanks, and all the best.
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Old 03-07-2012, 01:50 PM   #2
SeqAA
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If you are talking about mate pair, there are people that can go up to 40kb+. This is not something the average user will be able to accomplish.
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Old 03-07-2012, 06:29 PM   #3
andyding
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Thanks for your reply.
I was wondering if you can tell me what is the potential technical difficulties when constructing libraries with ultra-large insert size?
Thanks again.

Andy

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Originally Posted by SeqAA View Post
If you are talking about mate pair, there are people that can go up to 40kb+. This is not something the average user will be able to accomplish.
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Old 10-29-2012, 07:02 AM   #4
hawaii454-0
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Hi,
Does anyone have final libraries which show shoulder peaks on their larger insert libraries?

Thanks,
Anna.
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