Go Back   SEQanswers > Sequencing Technologies/Companies > Ion Torrent

Similar Threads
Thread Thread Starter Forum Replies Last Post
Need help identifying graph thing slash4x4 Bioinformatics 3 04-08-2016 12:01 AM
mapping graph pony2001mx Bioinformatics 0 09-17-2013 07:18 AM
Overlap Graph navin_elango Bioinformatics 2 05-20-2013 10:56 AM
Plot graph gopu_36 Bioinformatics 1 11-15-2009 11:37 PM

Thread Tools
Old 09-18-2019, 10:39 PM   #1
Junior Member
Location: turkey

Join Date: Sep 2019
Posts: 1
Default Torrent s5 pH graph

Hi there,
I am adding my pH graph after initialization (*Attach Files).
Are these values good ? pH values about 7,5 -and that is good- but I cannot sure about the graph.
What is your opinion? Thanks !
Attached Images
File Type: jpg IMG-20190919-WA0000.jpg (70.9 KB, 19 views)
missphelps is offline   Reply With Quote
Old 09-23-2019, 12:23 AM   #2
Junior Member
Location: Netherlands

Join Date: Jul 2019
Posts: 3

Hi missphelps,

Thermo Fisher recommends not to use the init plot as it can be open to misinterpretation. Saying that, your graph shows a typical reagent check profile with the nucs rising steeply before decreasing. The purple W2 line should also rise quite steeply before stabilizing.

If the graph deviates a lot from what I describe above (for instance when the signal is very messy or noisy), then it is recommended to repeat the reagent check from the System Tools menu. Often it will look better after repeating the reagent check. When the results don't improve, then reach out to the Thermo Fisher Technical Support team.
Bastiaan is offline   Reply With Quote
Old 09-27-2019, 11:15 AM   #3
Location: PharmLand -- Philadelphia

Join Date: Aug 2008
Posts: 13

Hello missphelps,
Hope you didn't waste a chip on that run. The signal differential between all of your nucleotides and the wash buffer should be close to 1000 or greater for any cartridge on the S5.

Good luck...
BioHak is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 05:08 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO