Hi everyone,
I would like to isolate some nuclei from some dikaryotic cells (2 nuclei per cell) and would like to use DAPI or Hoechst stain to label the nuclei. Does anyone know if these stains will interfere with downstream applications such as PCR or next-gen sequencing? I have tried using Giemsa stain but I am finding it really hard to identify the nuclei because clearing the cells after staining is quite difficult.
Thanks very much
Will J
I would like to isolate some nuclei from some dikaryotic cells (2 nuclei per cell) and would like to use DAPI or Hoechst stain to label the nuclei. Does anyone know if these stains will interfere with downstream applications such as PCR or next-gen sequencing? I have tried using Giemsa stain but I am finding it really hard to identify the nuclei because clearing the cells after staining is quite difficult.
Thanks very much
Will J