Hi,
I need advise since I'm not aware of SNPs area...
I observe 2 reads matching a mature miRNA which are most abundant ones:
1) first is perfect match with the mature with 200K counts
2) secong have 1 mismatch but have double read counts (400K) - more abundant than the perfect match!
I think this cannot be error since it is sequenced 400K times with the same mistmach? or I'm wrong?
Can I conclude that this can be SNP in the the miRNA or no?
Thanks
I need advise since I'm not aware of SNPs area...
I observe 2 reads matching a mature miRNA which are most abundant ones:
1) first is perfect match with the mature with 200K counts
2) secong have 1 mismatch but have double read counts (400K) - more abundant than the perfect match!
I think this cannot be error since it is sequenced 400K times with the same mistmach? or I'm wrong?
Can I conclude that this can be SNP in the the miRNA or no?
Thanks
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