Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • sff extract

    Hi
    How can I extract sff files? only in linux or windows is possible too?

  • #2

    This program will do the job. It works on Windows and linux so long as you have python installed.

    Comment


    • #3
      you can try:

      Usage: sffinfo [options...] [- | sfffile] [accno...]
      Options:
      -a or -accno Output just the accessions
      -s or -seq Output just the sequences
      -q or -qual Output just the quality scores
      -f or -flow Output just the flowgrams
      -t or -tab Output the seq/qual/flow as tab-delimited lines
      -n or -notrim Output the untrimmed sequence or quality scores
      -m or -mft Output the manifest text

      Comment


      • #4
        bio-x is suggesting using the Roche tool sffinfo which comes with the "Newbler" package. Note that this is Linux only. See also getting Newbler.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Essential Discoveries and Tools in Epitranscriptomics
          by seqadmin




          The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
          04-22-2024, 07:01 AM
        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Today, 08:47 AM
        0 responses
        10 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        60 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        57 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        53 views
        0 likes
        Last Post seqadmin  
        Working...
        X