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Old 10-09-2017, 12:26 PM   #1
Location: U.S.

Join Date: May 2017
Posts: 21
Angry Single kit for bacterial RNAseq projects (from clinical samples)?


Does anyone know of a standalone kit or protocol which could deplete mammalian rRNA and bacterial rRNA to be left with bacterial RNA we want for sequencing? The best way I know how seems to be a convoluted mess of ribozero, microbe enrich, and truseq by illumina while skipping the poly-A selection step. Surely there has to be a more straightforward way to achieve desired RNAseq experiments for clinical bacterial samples?
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Old 10-09-2017, 12:42 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
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If you want to use Truseq then you will need to do sequential depletion with microbial depletion reagent and use the eluate with RiboZero followed with the rest of protocol.
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Old 10-10-2017, 01:18 AM   #3
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Location: Dunedin, NZ

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Ribo-Zero Epidemiology kit, which combines Ribo-zero human/mouse/rat and bacteria in a single tube for a single depletion step.
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rna library, rnaseq, sample cleanup, sample preparation

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