SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Experiment deletion failing BenjaminL Ion Torrent 0 07-29-2012 11:10 PM
Need Help Designing Experiment. master_shake RNA Sequencing 0 07-17-2012 02:56 PM
How many tags to perform a RNAseq experiment kant1_IC RNA Sequencing 6 02-06-2012 07:18 AM
Any plan to upgrade the backend system? marcowanger Site Feedback/Suggestions 0 11-03-2011 01:23 AM
Minimum information about NGS Experiment wdt Bioinformatics 3 03-21-2011 01:24 PM

Reply
 
Thread Tools
Old 06-02-2013, 01:53 AM   #1
Nullie
Junior Member
 
Location: -

Join Date: Jun 2013
Posts: 9
Default Help to plan experiment

Hello everyone,

I am a biology student and I need to check the microbial population in the intestine before and after taking a certain medication, to see if the drug affects the microbe abundance in the intestine... I need to plan this experiment using NGS and I am LOST!
please can someone help me to plan this experiment...? what NGS technology should I use after extraction of DNA/RNA samples?

Thank You!
Nullie is offline   Reply With Quote
Old 06-03-2013, 05:47 AM   #2
microgirl123
Senior Member
 
Location: New England

Join Date: Jun 2012
Posts: 192
Default

Have you looked into 16s rDNA sequencing? It may not be the best solution, but there are lots of papers out there using this method.
microgirl123 is offline   Reply With Quote
Old 06-03-2013, 06:36 AM   #3
rhinoceros
Senior Member
 
Location: sub-surface moon base

Join Date: Apr 2013
Posts: 372
Default

1. Think very hard what biological questions you want to address
2. Find out how much funding you have
3. Read a ton of papers
4. Make an informed decision
rhinoceros is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 08:36 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO