Hi everyone,
Has anyone any advice about the minimum molecular weight needed for 150 bp PE read Illumina whole genome re-sequencing (coverage of 5x) at BGI?
I am also wondering whether it is better to have high DNA concentration (>200 ng/µl) and a smearing pattern or lower DNA concentration (20-40 ng/µl) of mostly high molecular weigth? Samples will be sequenced on an HiSeq X.
Thank you in advance for your help.
Marie
Has anyone any advice about the minimum molecular weight needed for 150 bp PE read Illumina whole genome re-sequencing (coverage of 5x) at BGI?
I am also wondering whether it is better to have high DNA concentration (>200 ng/µl) and a smearing pattern or lower DNA concentration (20-40 ng/µl) of mostly high molecular weigth? Samples will be sequenced on an HiSeq X.
Thank you in advance for your help.
Marie
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