Number of samples on a 316 chip?

[cmcquitty]

I would like to sequence the whole genome of several E.coli mutants, genome size 5.3MB, to look for single snps. (Using Ion torrent PGM) I have 8 samples in total, would it be realistic to run them all on a 316 chip?

Any advice would be great, I'm completely new to this!

[AllSeq]

The PGM 316 chip can generate around 1Gb of data. So for 8 samples with 5.3Mb genomes, you’ll get roughly 20X coverage of each genome. It depends on what you’re trying to do with the data, but that coverage sounds a little low. You could either bump up to the 318 chip to double your coverage, or run only 4 samples per 316 chip. Feel free to check out AllSeq’s NGS Knowledge Bank for details on the Ion Torrent and various other sequencing platforms to help you make these judgments for yourself. And of course, if you’re looking to outsource any of your projects, we can help you find the best provider.