We’re about to start targeted sequencing for a study with about 1500 samples (500 trios). The samples will be multiplexed at 96x per flow cell lane.
Does anyone have suggestions on how to randomize the samples in regards to sample prep/ flow-cell placement? Would you randomize by individual samples or by families (keeping trios together)? It may not affect results much, but I would like to avoid reviewer comments down the road.
Does anyone have suggestions on how to randomize the samples in regards to sample prep/ flow-cell placement? Would you randomize by individual samples or by families (keeping trios together)? It may not affect results much, but I would like to avoid reviewer comments down the road.
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