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  • what specific sequence is used for annealing to flow cell?

    I am designing my own adapters
    and need to know which specific sequence is actually used for the annealing to the flow cell.

    I understand it is there inside the PE PCR primers sequence but I 'd like to specifically know which part of it, to properly judge what can I change/retain.

    Thanks million for any suggestions!!

  • #2
    Hello,
    I think you can have a part of your answer here:

    Comment


    • #3
      Here is a diagram that I drew up of the TruSeq process. The bridge PCR sequences (the oligos attached to the flow cell) are shown at the bottom of the diagram.
      Attached Files

      Comment


      • #4
        Thanks!

        Thank you very much huguesparri and bbeitzel!!
        That is what I was looking for

        Comment

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