Tweet
For anyone who's used the Covaris, I have a question: can I store sheared gDNA in the same manner that one would store unsheared gDNA? I need to do long-term storage for some sheared samples. Currently they are frozen at -80 in nuclease-free water. Are there any other steps I should take?
-
-
I never stored sheared gDNA after Covaris. I just had the feeling that broken ends would facilitate the degradation by nucleases, even at -20 or -80. I always did the end-repair step as well. But this is not based on any literature, just personal feeling! :-)
In this way I stored samples for 6 months and afterwards they were fine. I believe you could store them even longer.
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment