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Old 10-09-2018, 11:56 PM   #1
ishmistry
Junior Member
 
Location: Oxford

Join Date: Oct 2018
Posts: 1
Default Single index adapters for low-plex

Hi,

I'm very new to sequencing so apologies if my question is naive.

I am trying to sequence whole human genome samples just to test the reproducibility of my library preparation method. I'm not interested in mutation frequency.

1. Would single-index adapters be good enough for pooling 6-8 samples, or do I need dual index?

2. What depth of coverage would I need to just see if my DNA is good enough to sequence. Could I use MiSeq or would I need HiSeq?

Thanks in advance for any help!
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hiseq, miseq, multiplex, single index, whole genome sequencing

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